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Fig. 5 | Cell & Bioscience

Fig. 5

From: Ammonium ions improve the survival of glutamine-starved hybridoma cells

Fig. 5

Non-additive effect of ammonium salts and α-ketoglutarate on the viability of Gln-starved Sp2/0-Ag14 cells. Cells were deprived of l-glutamine for 3 h in the presence of 2 mM dimethyl α-ketoglutarate (KG) and 5 mM ammonium salt/sodium salt or an equivalent volume of PBS. Cultures in which ethanol (Eth) was added in place of KG were included as controls. Gln (4 mM) was then added and culture was resumed for 24 h. Cell viability was determined by trypan blue exclusion. Cultures in which Gln was added at the start of the experiment were included as controls. *p < 0.05 vs corresponding ethanol-supplemented control. ŧ < 0.05 vs corresponding sodium salt-supplemented control

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