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Fig. 2 | Cell & Bioscience

Fig. 2

From: Ammonium ions improve the survival of glutamine-starved hybridoma cells

Fig. 2

Ammonium salts improve the viability of glutamine-starved Sp2/0-Ag14 cells—trypan blue exclusion assay. Cells were deprived of l-glutamine for 3 h in the presence of 5 mM ammonium salt/sodium salt/methylamine chloride or an equivalent volume of PBS. Cell viability was determined by trypan blue exclusion. Cultures in which Gln was added at the start of the experiment were included as controls. *p < 0.05 vs corresponding sodium salt-supplemented control

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