Fig. 4

Off-target effects of CRISPR/Cas9 in X. tropicalis evaluated via T7E1 assay. a Representative gel image of T7E1 digested off-target amplicons. The percentage under panels indicated the frequencies of off-target effects. b Quantification of the cleavage frequencies of all off-target sites. No clear and specific bands after T7E1 assay were generated at sox9-579 and lrig3-E306 loci. The cutting efficiencies at sox9-579 and lrig3-E306 sites were calculated by sequencing results. Red dash line indicated 10 % in indels efficiency