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Fig. 7 | Cell & Bioscience

Fig. 7

From: Cold temperature blocks thyroid hormone-induced changes in lipid and energy metabolism in the liver of Lithobates catesbeianus tadpoles

Fig. 7

Effects of 3,3′,5-triiodothyronine and cold exposures on gene transcripts for glycerophospholipid synthesis (A) and very long chain fatty acid desaturation and elongation (B). Reverse transcription-quantitative polymerase chain reaction analyses were carried out with RNAs from the liver of the tadpoles (n = 6/group), as described in Fig. 5. The genes tested are: glycerol kinase (gk), lysophosphatidic acid acyltransferase-α (lpaata), phosphate cytidylyltransferase 1 (pcyt1), phosphate cytidylyltransferase 2 (pcyt2), phosphatidylserine decarboxylase 1 (psd1), phosphatidylethanolamine N-methyltransferase (pemt), phospholipase D1 (pld1), phosphatidate cytidylyltransferase 1 (cds1), phosphatidate cytidylyltransferase 2 (cds2), belonging to the de novo synthesis pathway of glycerophospholipids; cytosolic phospholipase A2 IVB (cpla2), the transcription factor (pparg) and 1-acylglycerol-3-phosphate O-acyltransferase 3 (agpat3), belonging to the remodeling pathway (cycle of deacylatiom/reacylation) in A; stearoyl-CoA desaturase or Δ9 desaturase (scd1), Δ6 desaturase or fatty acid (FA) desaturase 2 (fads2), Δ5 desaturase or FA desaturase 1 (fads1), and the enzymes for elongation of very long chain FAs (elovl1, elovl2, elovl3, elovl5 and elovl6) in B. The data are mean ± SEM (n = 6). Different letters indicate significantly different means (p < 0.05). These experiments were repeated two times, with similar results

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