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Fig. 6 | Cell & Bioscience

Fig. 6

From: Cold temperature blocks thyroid hormone-induced changes in lipid and energy metabolism in the liver of Lithobates catesbeianus tadpoles

Fig. 6

Effects of 3,3′,5-triiodothyronine and cold exposures on energy metabolic gene transcripts. Reverse transcription-quantitative polymerase chain reaction analyses were carried out with RNAs from the liver of the tadpoles (n = 6/group), as described in Fig. 5. The genes tested are: phosphorylase, glycogen, liver (pygl), hexokinase (hk), aldolase B (aldb), phosphoglycerate kinase 1 (pgk1), lactate dehydrogenase B (ldhb), cytochrome c oxidase subunit IV (cox4), glucose-6-phosphatase, catalytic, 2 (g6pc2), malate dehydrogenase (mdh), phosphoenolpyruvate carboxykinase 1 (pck1) and fructose-1,6-bisphosphatase 2 (fbp2), belonging to the pathways of the glycolysis, TCA, electron transport system, and glyconeogenesis; diacylglycerol O-acyltransferase 1 (dgat1), acetyl-CoA carboxylase β (accb), FA synthase (fas), acetyl-CoA acyltransferase 2 (acaa2), long chain-acyl-CoA dehydrogenase, (acadl), carnitine palmitoyltransferase 1 (cpt1a) and ceramide synthase (cers), belonging to the pathways of the biosynthesis and metabolism of FAs, TGs and ceramides; 3-hydroxy-3-methyl-glutaryl-CoA reductase (hmgcr), sterol-C4-methyl oxidase–like (sc4mol), low density lipoprotein receptor 1 (ldlr1), cytochrome P450 7A1 (cyp7a1), cytochrome P450 8B1 (cyp8b1), 3β-hydroxy-Δ5-C27-steroid dehydrogenase type 7 (hsd3b7), sterol O-acyltransferase 1 (soat1), lecithin-cholesterol acyltransferase (lcat), hydroxysteroid (17-β) dehydrogenase 12 (hsd17b12), belonging to the pathways of the biosynthesis, metabolism and transport of cholesterols, steroids and bile acids; and the transcription regulatory proteins, which are peroxisome proliferator-activated receptor γ coactivator 1α (pgc1a), sterol regulatory element-binding transcription factor 1 (serbp1), sterol regulatory element-binding transcription factor 2 (serbp2) and peroxisome proliferator-activated receptor α (ppara). The data are mean ± SEM (n = 6). Different letters indicate significantly different means (p < 0.05). These experiments were repeated two times, with similar results

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