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Fig. 4 | Cell & Bioscience

Fig. 4

From: Type I interferon inhibits varicella-zoster virus replication by interfering with the dynamic interaction between mediator and IE62 within replication compartments

Fig. 4

IFN-α reduces nuclear MED25 expression and interferes with its redistribution to IE62-expressing replication compartments. Untreated or IFN-α treated HELF monolayers were fixed in 4 % PFA at 12 or 24 h post VZV inoculation. The cell monolayers were double stained with anti-MED25 (green) and anti-IE62 (red) antibodies for confocal microscopic analysis. Representative double immunofluorescence stained single cells from untreated (a) or IFN-α treated (b) illustrated MED25 expression in IE62-expressing replication compartments in small, large or large globular pattern. Arrows shown in merged images indicate colocalization of IE62 and MED25. c Expression of Med25 mRNA in untreated or IFN-α-treated HELFs at 12 and 24 h after incubation with VZV-infected or mock-infected cells was measured by qRT-PCR. The level of expression was normalized to the expression of human β-actin and expressed as relative expression to human β-actin. Each bar represents the mean ± SEM of triplicate measurements from one experiment. d MED25 integrated intensity from each cell was measured by MetaMorph software. The averages of MED25 integrated intensity in untreated (white bars) or IFN-α treated cells (black bars) without VZV inoculation or with VZV infection are shown in (d). MED25 integrated intensity expressed in the nucleus of uninfected HELF cells was measured based on DAPI stain. The averages of MED25 integrated intensity for IE62-expressing cells in various morphological forms in untreated or IFN-α treated cells are shown. Each bar represents the mean ± SEM of MED25 integrated intensity from 14–16 cells (untreated group) or 20–30 cells (IFN-α treated group) analyzed. Differences between each groups were tested by unpaired Student’s t test (**p < 0.01; ***p < 0.001; not significant (n.s.), p > 0.05). Scale bar 5 μm

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