Fig. 1

Dose dependent effects of IFN-α on IE62 expression. Primary human embryonic fibroblasts (HELFs) were untreated or treated with IFN-α at 300, 1000 or 10,000 U/ml for 24 h followed by inoculation of green fluorescent dye CFSE-labeled VZV infected cells. a The cell monolayer was fixed at 24 h, stained with primary antibody to VZV IE62 and detected with donkey anti-rabbit IgG (red). Green stained cells were inoculated VZV-infected cells. Cells were counterstained with DAPI and analyzed by confocal microscopy. Shown are representative illustrations from at least three independent experiments. Scale bar 100 μm. b IE62 positive cells were counted for each group of cells in the total of 20–200 cells in the same coverslip. The percentages of cells that expressed IE62 were thus calculated. Shown is the average from two independent experiments with error bars