Fig. 2

Baicalein induced apoptosis in 6133 MPL/W515Lcells. a 6133 MPL/W515L cells were treated with or without baicalein (0, 10, and 20 µM) for 12 h and subjected for Annexin V staining (PE-conjugated antibody) to measure apoptosis. b The treated cells were collected for western blot to detect cleaved caspase 3 as an apoptotic marker. Hsc70 serves as a loading control. c 6133 MPL/W515L cells were treated with or without baicalein and Z-VAD in combination as indicated for 24 h. The treated cells were collected for western blot to detect cleaved caspase 3 as an apoptotic marker. d 6133 MPL/W515L cells were treated with or without baicalein (BAI, 10 µM) and Z-VAD (10 µM) in combination as indicated for 24 h. The treated cells were collected for Annexin V staining to measure apoptosis. e The treated cells numbers were counted after 24 h (day 1) and 48 h (day 2) and the proliferation rate was presented. Data were statistics (Mean ± SD) of a representative experiment (triplicates) from three independent experiments with similar results. *p < 0.05, **p < 0.01and ***p < 0.001 as compared to vehicle treatment