Fig. 6
pNGVL4a-Sig/E7(detox)/HSP70 DNA priming followed by TA-CIN boost generated strong therapeutic anti-tumor effects in TC-1 tumor-bearing mice. Five to eight weeks old female C57BL/6 mice (5 mice/group) were injected with 1 × 105 of TC-1 tumor cells subcutaneously. Three days later, one group of the tumor-bearing mice was vaccinated with 25 μg/mouse of pNGVL4a-Sig/E7(detox)/HSP70 DNA in 50 μl three times. Another group of mice was vaccinated with 25 μg/mouse of pNGVL4a-Sig/E7(detox)/HSP70 DNA in 50 μl twice followed by single 25 μg/mouse of TA-CIN protein in 20 μl. All vaccinations were given via intramuscular injection (leg muscle) with 4-day interval. a Schematic illustration of the experiment. b Summary of tumor volume. c Kaplan–Meier survival analysis of TC-1 tumor-bearing mice