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Fig. 5 | Cell & Bioscience

Fig. 5

From: Restored expression of vitamin D receptor and sensitivity to 1,25-dihydroxyvitamin D3 in response to disrupted fusion FOP2FGFR1 gene in acute myeloid leukemia cells

Fig. 5

Expression of FOP2FGFR1 and presence of phospho-STAT1 in KG1, KG1-CtrA and KG1-RARA cells. The expression of wild-type FGFR1, wild-type FOP2 and the fusion FOP2FGFR1 gene in KG1, KG1-CtrA and KG1-RARA cells was tested by Real-time PCR relative to GAPDH expression levels (a). The expression levels obtained for KG1 cells were calculated as 1. The bar charts show mean values of three experiments (±SEM) of relative quantity (RQ). Values that differ significantly (p < 0.05) from those obtained for KG1 cells are marked with asterisks. The region between exon 4 of FOP2 and exon 10 of FGFR1 within the fusion FOP2FGFR1 gene was amplified from genomic DNA obtained from KG1, KG1-CtrA, KG1-RARA and KG1-Ctr-len cells (b). The PCR products were separated in 1 % agarose gel and visualized with ethidium bromide under UV light. Total cell lysates from KG1, KG1-CtrA and KG1-RARA cells were analyzed in western blots for the presence of active STAT1 and total STAT1 level (c). Actin content was tested as a control of equal loading and transfer of proteins. The experiment was repeated two times, each in duplicate, and illustrative blot is presented

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