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Fig. 4 | Cell & Bioscience

Fig. 4

From: Restored expression of vitamin D receptor and sensitivity to 1,25-dihydroxyvitamin D3 in response to disrupted fusion FOP2–FGFR1 gene in acute myeloid leukemia cells

Fig. 4

Accessibility of VDR and GAPDH proximal promoters in HL60, KG1 and KG1-CtrA. HL60, KG1 and KG1-CtrA cells were treated with nucleases in order to stimulate chromatin digestion after which chromatin was isolated, followed by Real-time PCR. The difference in CT values between digested and undigested chromatin allowed for evaluation of chromatin states (% chromatin accessibility) among the cell lines. GAPDH was used as the control (constitutively expressed) and RHO was used as the reference (epigenetically silenced) gene. Data are the mean values (±SEM) of three individual experiments

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