Fig. 2
miR-690 synergistically potentiates Runx2-induced osteogenic differentiation. a Real-time qPCR analysis of miR-690 was performed in Dox-treated samples used for miRNA profiling analysis. b, c Effect of miR-690 on expression of osteoblastic markers. C2C12/Runx2Dox cells were transfected with miR control (b), miR-690 (b), anti-miR control (c), and anti-miR-690 (c), respectively, and then treated with Dox for 3 days. The mRNA level of Alp and OC was measured by real-time qPCR. *P < 0.05 compared with miR control or anti-miR control samples. d Effect of miR-690 on ALP activity. C2C12/Runx2Dox cells were transfected with miR control and miR-690, respectively, and then treated with Dox for 7 days. The ALP activity was determined by ALP staining. Similar results were obtained in three independent experiments