Specific activity of respiratory complex-III and of citrate synthase in amastigotes isolated from macrophages | ||||||
---|---|---|---|---|---|---|
Enzymes | Control (WT) | WT + ANTIMYCINA (72 h) | LdU−/+ | LdU−/+ AB | ||
24Â h | 48Â h | 72Â h | 72Â h | |||
ComplexIII activity/min/mg protein | 110.4 ± 9.3 | 26.4 ± 1.8 | 45.3 ± 3.2 | 19.4 ± 1.9 | 8.2 ± 0.8 | 104 ± 9.1 |
Citrate synthase activity | 492.5 ± 26.2 | – | 452 ± 23.1 | 416 ± 19.8 | 46 ± 2.8 | 478.2 ± 24.2 |