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Fig. 5 | Cell & Bioscience

Fig. 5

From: Knockdown of CLIC4 enhances ATP-induced HN4 cell apoptosis through mitochondrial and endoplasmic reticulum pathways

Fig. 5

Effect of CLIC4 on the Ca2+ store release. Representative traces (a and c) and summarized data (b and d) showing 100 μmol/L ATP− (a and b) and 2 μmol/L thapsigargin (TG, c and d) -induced HN4 cell intracellular Ca2+ ([Ca2+]i) rise in Ca2+-free saline solution. [Ca2+]i was detected by the cell-permeable Ca2+-sensitive fluorescent indicator Fluo-8 AM. The change in [Ca2+]i was indicated by the ratio of the fluorescence density before and after the application of agonists. The HN4 cells were transfected with CLIC4 (CLIC4 siRNA) or scrambled (Con) siRNA. Values are shown as the mean ± SE. n = 5. *P < 0.05. vs. the control (Con) group

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