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Fig. 5 | Cell & Bioscience

Fig. 5

From: Probing the nuclear import signal and nuclear transport molecular determinants of PRV ICP22

Fig. 5

Investigation of the interactions between P-ICP22 and importin β1, transportin-1, importin α5, importin α1, importin α3 or importin α7. Co-IP of P-ICP22 with importin β1 (a), importin α5 (b), Transportin-1 (c), importin α1 (d), importin α3 (e) or importin α7 (f). HEK293T cells were co-transfected with pP-ICP22-EYFP and pCMV9-3 × Flag-importin β1 (3 × Flag-importin β1), pcDNA-Flag-kα1 (Flag-importin α5), pFLAG-CMV-transportin-1 (Flag-transportin-1), Flag-importin α1, Flag-importin α3 or Flag-importin α7. 24 h after transfection, cells were lysed and IP with anti-Flag or mouse IgG control. Immunoprecipitated proteins as well as the cell lysates were separated in denaturing 10 % SDS-PAGE, and analyzed by IB with anti-Flag mAb or anti-YFP pAb

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