Fig. 5

Antibodies against α9 and β1 integrin subunits block KSHV nucleocapsid trafficking to the perinuclear region by 30 min PI. A HFF cells were untreated or pre-treated (1 h at 37 °C) with antibodies (20 µg/ml) to α9, β1, α5 or a pre-immune IgG prior to infection with KSHV for 30 min at 37 °C. Cells were washed, fixed, and subsequently immunostained for TRI-1 minor capsid protein. Representative fluorescence imaging depicting TRI-1 positive viral capsids in infected HFF cells are shown. Mag ×1000. B Statistical analysis on the viral capsids that accumulated around the perinuclear region was determined based on randomly counting the number of TRI-1 positive KSHV capsids around 10 different nuclei. The TRI-1 positive viral capsids around the perinuclear region for different treatments is presented as percentage (%) as compared to cells infected with KSHV alone (n = 60). Data represent the average ±SD (error bars) of three experiments. Columns with different alphabets are statistically significant (p < 0.05) by LSD