Fig. 8From: Molecular and cytological analyses reveal distinct transformations of intestinal epithelial cells during Xenopus metamorphosisEpithelial apoptosis takes place prior to the appearance of clusters (islets) of proliferating adult intestinal stem cells during natural metamorphosis. Tadpoles at premetamorphic stage 54 (A), climax (B stage 60, C stage 62), and end of metamorphosis (D stage 66) were injected with EdU 1 h before being sacrificed. Cross-sections of the intestine from the resulting tadpoles were double-stained for apoptosis by TUNEL and for EdU. Higher magnifications of boxed areas in (A–D) are shown in (a′–d′). The dotted lines depict the epithelium-mesenchyme boundary (see Fig. 1)Back to article page