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Fig. 3 | Cell & Bioscience

Fig. 3

From: Molecular and cytological analyses reveal distinct transformations of intestinal epithelial cells during Xenopus metamorphosis

Fig. 3

The newly formed proliferating adult intestinal epithelial cells during T3-induced metamorphosis have little or no expression of IFABP. Premetamorphic stage 54 tadpoles treated with 10 nM T3 for 0 (A), 3 (B), or 6 days (C) and were sacrificed 1 h after injection with EdU. Cross-sections of the intestine from the resulting tadpoles were double-stained for IFABP by immunohistochemistry and for EdU. Higher magnifications of boxed areas in (AC) are shown in (a′–c′) and (a″–c″). The dotted lines depict the epithelium-mesenchyme boundary (see Fig. 1). Note that the EdU labeling revealed profound cell proliferation after T3 treatment. The proliferating cells in the epithelium after 6 days of T3 treatment were present mainly in clusters where IFABP staining was weak or absent (C, c″)

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