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Fig. 4 | Cell & Bioscience

Fig. 4

From: A new design of a lentiviral shRNA vector with inducible co-expression of ARGONAUTE 2 for enhancing gene silencing efficiency

Fig. 4

Co-expression of AGO2 enhances shRNA-mediated knockdown of DsRed2 florescence reporter. a Design of a set of shRNAs targeting DsRed2 coding region at different positions; b a schematic illustration of a shRNA lentiviral vector with CMV promoter-driven AGO2 (shRNA/AGO2). The corresponding control (shRNA/control) was generated by removing AGO2 fragment; c U6-driven shRNA with the co-expression of CMV-driven AGO2 or its control in the single plasmid were co-transfected with EGFP/DsReds plasmids into HEK293. Two days after transfection, R/G fluorescence intensity ratio was measured by flow cytometry analysis and the silencing efficiency was normalized to the shCon/control group. The results shown are mean ± SD from four independent experiments. ***P < 0.001

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