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Fig. 2 | Cell & Bioscience

Fig. 2

From: A new design of a lentiviral shRNA vector with inducible co-expression of ARGONAUTE 2 for enhancing gene silencing efficiency

Fig. 2

Dose-dependent analysis of AGO2 overexpression on RNAi efficiency. a Dose-dependent effects of AGO2 overexpression on EGFP silencing. HEK293 cells were co-transfected with pENTR/CMV-EGFP, pDsRed2-c1, shEGFP450 and different amount of AGO2 overexpression vector or the control vector without AGO2 insert. Representative images were shown from the cells 2 days after transfection; b the effect of AGO2 overexpression on EGFP silencing activities was quantified by analyzing G/R fluorescence intensity ratio. The results shown are mean ± SD from four independent experiments. ***P < 0.001 vs. control

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