Fig. 5

G-CSF treatment deteriorates the TBI-induced increase in ROS production in HSCs. Mice received vehicle or G-CSF treatment twice a day for 7 days after exposure to 0 or 4 Gy TBI as illustrated in Fig. 1a. BM-MNCs were harvested from scarified mice 1 month after exposure to TBI. The ROS production in HPCs and HSCs were analyzed. a Flow cytometric analysis was used to to estimate the ROS production in HPCs and HSCs. b The levels of intracellular ROS fold expression in HPCs are presented as the mean ± SE of DCF MFI. c The levels of intracellular ROS fold expression in HSCs are presented as the mean ± SE of DCF MFI