Fig. 4

G-CSF exacerbated the HSCs long-term and multilineage engraftment reduction induced by TBI. a Competitive transplantation assay were performed by transplanting a 4:1 ratio of 45.1 donor cells to 45.2 competitive cells into 9.5 Gy-irradiated recipients. After stable hematopoietic reconstitution (16 weeks), mice were sacrificed, and bone marrow cells were harvested and transplanted into secondary recipients in the same way as the first competitive assay. A peripheral blood chimera for total cells, T cells, and B cells was assessed after 16 weeks. b Representative flow cytomerty plots showing donor-derived cell engraftment in peripheral blood cells. Right: percentage of donor (CD45.1+) cell engraftment. Left: engraftment of donor-derived T cells (CD45.1 + CD3 + cells), B cells (CD45.1 + B220 + cells), and myeloid cells (CD45.1 + CD11 + and/or Gr-1 + granulocyte–monocyte–macrophage) in the peripheral blood. c Donor -derived bone marrow cells multilineage engraftments were determined at 8 weeks in lethally irradiated recipients after first transplantation. d Donor -derived bone marrow cells multilineage engraftments tendency were measured at 8 and 16 weeks in lethally irradiated recipients after first transplantation. e Donor -derived bone marrow cells multilineage engraftments were determined at 16 weeks in lethally irradiated recipients after second transplantation of BM-MNCs from mice receiving various treatments described in Fig. 1a. Results are presented as mean ± SE. * p < 0.05, ** p < 0.005 vs. control; ^# p < 0.05, ^## p < 0.005 (vs. 4 Gy) (N = 9–12 recipient mice/group)