SIRT1 inhibits adipogenesis and promotes myogenic differentiation in C3H10T1/2 pluripotent cells by regulating Wnt signaling

Table 1 Primer sequences in this study

Gene	Primers (sense/antisense 5′–3′	Product length (bp)	Ta (°C)
MyoD	CGCTCCAACTGCTCTGATGGCAT	280	61
MyoD	GGGTTCCCTGTTCTGTGTCGCTT	280	61
MyHc	TCGGGGTCTTGGACATTGCTGG	342	61
MyHc	CTTTGGCGGGTTTGGGCTTCTG	342	61
PPARγ	TGGGTGAAACTCTGGGAGATTC	250	60
PPARγ	AGAGGTCCACAGAGCTGATTCC	250	60
aP2	GTGTGATGCCTTTGTGGGAAC	235	60
aP2	CCTGTCGTCTGCGGTGATT	235	60
adiponentin	GCTCTCCTGTTCCTCTTAATCCT	437	60
adiponentin	CCAGTGCTGCCGTCATAATG	437	60
Cyclin D1	AAATGCCAGAGGCGGATGAG	200	60
Cyclin D1	AAGAAAGTGCGTTGTGCGGT	200	60
sFRP2	AATCGGCATCTAAGTCTT	129	56
sFRP2	GCAATGAGGAATGGTTAC	129	56
DACT1	TCTGAGGAATGGAAGTGTG	169	58
DACT1	GTCTGTCTTTGAGTCTTTGG	169	58
β-actin	GGCACCACACCTTCTACAATG	133	60
β-actin	GGGGTGTTGAAGGTCTCAAAC	133	60

ISSN: 2045-3701