Fig. 4
Effect of SIRT1 activation/inhibition on myogenic commitment and adipogenic commitment of MSCs. a Schematic representation of the experimental design: 50 μM resveratrol or 10 mM nicotinamide was added to C3H10T1/2 cells with 20 μM 5-AZA during commitment for 3 days, then C3H10T1/2 cell was cultured in GM for 14 days. b Cells were fixed and stained with Oil Red-O on day 14 days after GM culture. c The quantification of b. The Oil-Red O stain was extracted with 100 % isopropanol and the optical density at 500 nm (OD500) was determined. d The relative expression profile of adipogenic marker genes: PPARγ, aP2 and adiponentin were determined using Real-time PCR. e PPARγ, aP2 and adiponentin protein expression detected by western blotting (left) and densitometry analysis (right) on day 14 days after GM culture. f Cells were fixed and stained with Dil on day 14 days after GM culture. g The relative expression profile of myogenic marker genes: MyoD and MyHc were determined using Real-time PCR. The red arrow points to the lipid droplet. Data represent mean ± SEM from three independent experiments. *P < 0.05; **P < 0.01