Fig. 2

5-Azacytidine induces C3H10T1/2 cells into differentiated adipocytes for different treatment time. The C3H10T1/2 cells were induced with 20 μM 5-azacytidine for 0, 12, 24, 48, 72, and 96 h, then changed adipocyte differentiation medium for 8 days. a After 8 days of culture in adipocyte differentiation medium, the cells were stained with Oil Red-O. b The quantification of a. The Oil-Red O stain was extracted with 100 % isopropanol and the optical density at 500 nm (OD₅₀₀) was determined. c Real-time PCR analyzed PPARγ, aP2 and adiponentin at 8 days after adipogenic medium stimulation. The red arrow points to the lipid droplet. Data represent mean ± SEM from three independent experiments. *P < 0.05; **P < 0.01