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Fig. 1 | Cell & Bioscience

Fig. 1

From: SIRT1 inhibits adipogenesis and promotes myogenic differentiation in C3H10T1/2 pluripotent cells by regulating Wnt signaling

Fig. 1

5-Azacytidine induces C3H10T1/2 cells into differentiated adipocytes and myocytes. C3H10T1/2 cells were treated with 5, 10, 20, and 40 μM 5-azacytidine for 3 days, then changed GM culture treated for next 14 days. a Cells were fixed and stained with DiI on day 14 days after GM Culture. b The relative expression profile of myogenic marker genes: MyoD and MyHc were determined using Real-time PCR at 0, 3, 7, and 14 days after treatment. c Oil Red-O staining of cells on day 14 days after GM culture. d The relative expression profile of adipogenic marker genes: PPARγ and adiponentin were determined using Real-time PCR at 0, 3, 7, and 14 days after treatment. The green arrow points to the myotube. The red arrow points to the lipid droplet. Data represent mean ± SEM from three independent experiments. *P < 0.05; **P < 0.01

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