Unveiling the hidden function of long non-coding RNA by identifying its major partner-protein

Cell & Bioscience

Table 1 Summary of lncRNA analyzed by several biochemistry approaches

LncRNAs	RBP	Biological function	Methods	Ref
ANRIL	PCR2, PCR1	Influences p16 ^INK4a gene expression and cell senescence	CLIP	[38]
Air	G9a	Targets G9a in cis for imprinting	CLIP	[57]
Fendrr	PRC2, WDR5	Regulates gene in cis and in trans	RIP	[29]
FIRRE	hnPNPU	Modulation of nuclear architecture across chromosomes	RAP	[58]
HOTAIR	PCR2, LSD1	Silences transcription in trans via its modular architecture	RNA pull-down	[37]
Hottip	MLL-WDR5	Activates gene expression via chromosomal looping	RIP	[59]
lincRNA-p21	hnRNP-K	Mediates p53-dependent gene repression	RNA pull down-MS	[31]
MALAT1	PSPC1, PSF, PURA	Sequesters splicing factor to regulate alternative splicing	CHART-MS	[60]
NEAT1	PSPC1, SRSF1, ESRP2	Plays roles in RNA processing and transcriptional regulation	CHART-MS	[60]
Rox1	MLE, MSL	Mediates X chromosome upregulated to rescue male lethality	dChIRP	[61]
TERC	TCAB1	Functions as the template and scaffold for the telomerase complex	ChIRP	[47]
Xist	81 proteins (Hnrnpk, Spen)	Mediates chromatin modifications and Polycomb targeting	ChIRP-MS	[62]
Xist	10 proteins (SHARP, HDAC3)	Interacts directly with SHARP to silence transcription through HDAC3	RAP-MS	[63]

ISSN: 2045-3701