Fig. 5

hMYH is a key factor for the interaction between hTopBP1 and hRad9. a HEK293 cells transfected with siGFP or siMYH were either untreated or treated with 20 mM HU for 1 h. The cell lysates were subjected to IP with an anti-hRad9 antibody. Immunoblotting was performed using the indicated antibodies. The bar graph shows the quantification of the interaction between hRad9 and hTopBP1. The values shown represent the mean ± standard error (of three independent experiments). b Cells overexpressing c-Myc or c-Myc-hMYH were either untreated or treated 20 mM HU for 1 h. Then, an IP assay was performed with an anti-hRad9 antibody. Immunoblotting was then performed as indicated. Quantification of the association between hRad9 and hTopBP1 is shown as mean ± standard error (of three independent experiments). P values were calculated using a paired t test, *p < 0.05