Table 1 Primers used for quantitative reverse-transcriptase polymerase chain reaction (RT-PCR)
Genes | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
m-PAX2 | AAGCCCGGAGTGATTGGTG | CAGGCGAACATAGTCGGGTT |
m-WT1 | CAGTTCCCCAACCATTCCTT | AAGCTGGGAGGTCATTTGGT |
m-Brachyury (Bry) | ACCCAGACTCGCCCAATTTT | GGAAAGCAGTGGCTGGTGAT |
m-CD24 | GTTGCACCGTTTCCCGGTAA | CCCCTCTGGTGGTAGCGTTA |
m-Osr1 | ATTCACCCTAAGCCAGAGATCA | AGGCCAGGTTAGCGAAATCAA |
m-Six2 | CTCACCACCACGCAAGTCAG | CTCGGAACTGCCTAGCACC |
m-Sall1 | CTCAACATTTCCAATCCGACCC | GGCATCCTTGCTCTTAGTGGG |
m-E-cad | CTCCAGTCATAGGGAGCTGTC | TCTTCTGAGACCTGGGTACAC |
m-AQP1 | AGGCTTCAATTACCCACTGGA | GTGAGCACCGCTGATGTGA |
m-PDGFR | CCACACCCTTGGGGAATAGT | TTTGAAGGGCAAGGAATGTG |
m-GAPDH | ACTTCAACAGCAACTCCCACTC | TAGGCCCCTCCTGTTATTATGG |
r-IL-10 | TAACTGCACCCACTTCCCAGT | TGGCAACCCAAGTAACCCTTA |
r-bFGF | CGACCCACACGTCAAACTAC | CCAGGCGTTCAAAGAAGAAA |
r-TGF-β1 | GCCCTGGATACCAACTACTGCT | GTTGGTTGTAGAGGGCAAGGAC |
r-EGF | CGTCTCAGTGGTCATGGATTT | TCAGAAGAACACGGGAATTGT |
r-HGF | CTCTCGTTCCTTGGGATTATTG | GATCCCCCACAGATGTGTTTAT |
r-PDGF | ATCGAGCCAAGACACCTCAAAC | ATGGGGGCAGTACAGCAAATAC |
r-GAPDH | AGTGCCAGCCTCGTCTCATAG | CGTTGAACTTGCCGTGGGTAG |