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Fig. 3 | Cell & Bioscience

Fig. 3

From: Molecular characterization of HIV-1 genome in fission yeast Schizosaccharomyces pombe

Fig. 3

Effects of HIV-1 viral proteins on colony formation. Wild type S. pombe cells containing HIV-1 viral gene-expressing plasmids were grown overnight in EMM liquid medium supplemented with thiamine to mid-log phase. The following day, cells were recovered, re-suspended in fresh media and streaked onto the minimal selection EMM agar plates with thiamine (left +T plates; no gene expression) or without thiamine (right −T plates; with gene expression). The plates were incubated at 30°C for 6 days before observation. The pYZ1N-vpr and pYZ1N plasmids were used as positive and negative controls, respectively [34].

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