Subcellular localization of HIV-1 proteins in fission yeast. Fission yeast strains expressing normal GFP or N-terminally GFP-tagged HIV-1 proteins were grown to a log phase in EMM selective media. The cells were re-inoculated into fresh media without thiamine (to induce gene expression) and grown for 24–30 h. The nuclei were stained with DAPI. The cells were examined using fluorescence microscopy for subcellular localizations of the GFP-tagged proteins with stained cellular nuclei. Each column represents different microscopic views: GFP, for protein subcellular location; DAPI, for localization of the nucleus; Merge, merging images of GFP and DAPI; and the “Contrast” is for the overall view of the cell. The Gag gene products are shown in a, the Pol and Env gene products in b, and the auxiliary and regulatory proteins in c. The scale bar represents 10 μm.