PLZF and UTF1 are regulated by the PI3K/PTEN signal pathway. a Western blot analysis of GFRα1, PLZF, UTF1, PTEN, and P-AKT expression in Pten
+/+ and Pten
−/− testes at 7 and 10 days old. b Western blot analysis of PLZF and UTF1 expression in cultured SSCs isolated from 7 day-old testes. The treatment with PI3K inhibitor or rapamycin lasted for 96 h, DMSO was used as control. c Coimmunofluorescent staining of PLZF and UTF1 in SSCs treated with PI3K inhibitor, rapamycin or DMSO, showed that the PI3K signal pathway inhibition induced PLZF but reduced UTF1 expression (scale bar is 20 µm).