Reduction of GFRα1 and PLZF positive SSCs and increase of UTF1 positive cells in neonatal Pten
−/− testes. Staining of 7 day-old testis sections showed the reduction of both GFRα1+ SSCs and PLZF+ SSCs a and the emergence of PLZF−/UTF1+ SSCs in the Pten
−/− testis (b, arrow). Staining of 10 day-old testis sections showed a significant loss of both GFRα1+ SSCs and PLZF+ SSCs (c) and an increase in the number of PLZF−/UTF1+ cells in the Pten
−/− testis (d, arrows). Nuclei were counterstained with DAPI (scale bar is 20 µm). Based on coimmunofluorescent staining of testicular tubule sections from both Pten
+/+ and Pten
−/− mice at 7 days old (e–g) and at 10 day olds (h–j), the average number ± SEM of GFRα1+, PLZF+ and PLZF−/UTF1+ cells per tubule cross-section were calculated and presented as bar graphs. For the statistical analysis, each five inconsecutive testis sections were counted. Horizontal bar indicates mean value, n = 4, *P < 0.05, **P < 0.01.