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Fig. 1 | Cell & Bioscience

Fig. 1

From: PTEN signaling is required for the maintenance of spermatogonial stem cells in mouse, by regulating the expressions of PLZF and UTF1

Fig. 1

Cre-recombinase mediated deletion of Pten in SSCs. a PCR analysis of genotype of Pten knockout (KO) mice. Short arrows denote the predicted size of Pten KO and wild-type (WT) alleles, long arrows denote the predicted size of Cre and internal positive control (+/+, wild type; +/−, heterozygote; −/−,homozygote; M, Marker). b Immunostaining of PTEN in cross sections of 7 day-old testis. The cytoplasmic staining was observed in spermatogonial cells from Pten +/+ section, whereas it was absent in the Pten −/− section (scale bar is 20 µm). c Western blot analysis of PTEN expression in whole testis of 32 day-old Pten +/+, Pten +/−, and Pten −/−mice. PTEN is also expressed in nongerminal cells, so PTEN expression was detected in Pten −/− testes, but was significantly lower compared with Pten +/+ and Pten +/− testes. d Absence of PTEN expression in haploid cells from Pten −/− testes. Haploid sperms were isolated from 79 day-old testes of Pten +/+ and Pten −/− mice separately by sorting using flow cytometer, then were subjected to Western blotting analysis.

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