Fig. 6

Acridine orange staining after partial- and complete glutamine- and glucose starvation for short term exposures and after 7 days. Acridine orange staining of HeLa cells (a) and MDA-MB-231 (b) after exposure to media presenting with different metabolic conditions. Acridine orange production did not change in any statistically significant manner when exposed to these metabolic conditions (P-value > 0.05). Acridine orange staining and colony formation after 7 days in HeLa (c) and MDA-MB-231 cells (d). Results demonstrated that glucose-and glutamine deprivation effects cell functioning days after exposure was terminated with all the exposure to conditioned media demonstrating increased acridine orange staining suggesting an increase in acidity. An asterisk (*) indicates P-value < 0.05