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Fig. 3 | Cell & Bioscience

Fig. 3

From: Influence of partial and complete glutamine-and glucose deprivation of breast-and cervical tumorigenic cell lines

Fig. 3

Cell cycle progression after partial- and complete glutamine- and glucose starvation for short term exposures and after 7 days. Percentage of cells occupying each cell cycle phase after cells was propagated in media according to metabolic state for the appropriate exposure period (2 h, 4 h and 6 h). Hela cells propagated in medium containing 6 mM glucose and 1 mM L-glutamine demonstrated an increased sub-G1- and G2M faction. MDA-MB-231 cells propagated in medium containing 6 mM glucose and 1 mM L-glutamine also showed an increased sub-G1 fraction. MDA-MB-231 and HeLa cells propagated in medium containing 6 mM glucose and 1 mM L-glutamine demonstrated an increased sub-G1 and G2M fraction. HeLa- and MDA-MB-231 cells propagated in media containing no glucose or L-glutamine demonstrated an increase in the number of apoptotic cells and the HeLa cells also showed an increase in the G2M fraction. The effects on the cell cycle and apoptosis induction on day 7 by means of flow cytometry using propidium iodide staining showed significant induction of apoptosis in all treated samples. Hela- and MDA-MB-231 cells propagated in medium containing 6 mM glucose and 1 mM L-glutamine 7 days before also demonstrated an increase in the number of cells occupying the S phase. The MDA-MB-231 cells also showed a G2M increase. The HeLa cells propagated in medium containing 3 mM glucose and 0.5 mM L-glutamine also presented with increased G2M fraction. MDA-MB-231 cells propagated in DMEM containing 0 mM-3 mM glucose and 0 mM-0.5 mM l-glutamine demonstrated an increased number of cells in the S phase. The Hela cells propagated in medium containing 0 mM glucose and 0 mM L-glutamine for 2 h and 4 h also showed an increased S phase. An asterisk (*) indicates P-value < 0.05

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