Fig. 2

Unsuccessful rescue of HeLa and MDA-MB-231 after 7 days. HeLa and MDA-MB-231 cells propagated in media according to metabolic state for 2 h, 4 h and 6 h, 7 days ago, which after cells were washed and medium was replaced with DMEM containing 6 mM glucose and 1 mM l-glutamine. The effects of the decreasing l-glutamine and glucose concentrations were more pronounced 7 days after the exposure when compared to those on the day of exposure self. Effects on decreased cell density were also more severe. Morphological characteristics observed included decreased cell density, reduced cell size and elongation. All of the above-mentioned morphological characteristics were more prominent in the DMEM containing 0 mM glucose and 0 mM l-glutamine when compared to cells propagated in DMEM containing 3 mM-6 mM glucose and 0.5 mM-1 mM l-glutamine. The same applies for the 6 h exposures compared to the 2 h and 4 h exposures. However, all of the above-mentioned effects appeared earlier in the MDA-MB-213 cell line when compared to the HeLa cell line (20x magnification). The scale bar in all images represents 50 μm