Fig. 1

Characterization of antigen-specific CD8⁺ T cell immune responses generated by antigen-specific DNA vaccine mixed with vectors containing BPV1 L1 or L2 DNA. C57BL/6 mice (five per group) were vaccinated intradermally via gene gun with 2 μg/mouse of CRT/E7 or OVA DNA with or without BPV1 L1 or L2 DNA twice at 1-week intervals. Splenocytes from vaccinated mice were collected 1 week after last immunization and the E7 or OVA-specific T cell immune responses were characterized using intracellular cytokine staining followed by flow cytometry analysis. a Representative flow cytometry data depicting the number of E7 (upper panel) or OVA(lower panel)-specific CD8⁺ T cells. b Bar graph representing the number of E7 (upper panel) or OVA (lower panel) -specific CD8⁺ T cells/3x10⁵ splenocytes (mean ± SD). Data shown are representative of two experiments performed. * indicates p < 0.05