Fig. 1
From: Peli1 negatively regulates type I interferon induction and antiviral immunity in the CNS
Peli1 negatively regulates TLR-mediated IFN-β induction in microglia. a-c QPCR analysis of relative mRNA expression for the indicated genes in WT and Peli1-KO bone marrow-derived macrophages (BMDM) (a) or microglia (b,c) stimulated with the indicated ligands for different TLRs: TLR4 (LPS, 100 ng/ml), TLR9 (CpG, 2.5 μM), TLR7/8 (R837, 1 μg/ml), TLR1/2 (Pam3CSK4, 1 μg/ml), and TLR3 (poly(I:C), 10 μg/ml). *P < 0.05 and **P < 0.01. d IB analysis of phosphorylated (P-) and total TBK1 and IKKε in whole-cell lysates of WT or Peli1-KO microglia stimulated with LPS (100 ng/ml)