Fig. 2

Ruxolitinib and CAPE block HD-Adv gene therapy mediated activation of NK cells by viral vector activated macrophages. NK-92 cells were deprived from IL-2 for 48 h and THP-1 cells were activated by 5 ng/ml PMA to prepare mature macrophages. Mature macrophages were harvested and cultured with NK-92 cells (1:1 ratio) in presence and absence of ruxolitinib or CAPE, and in presence of both the inhibitors. As depicted in figure two cohort of these culture conditions were transduced with HD-Adv and other cohort was not transduced. After culturing them for 24 h, total RNA was isolated and analysed by qRT- PCR to look for relative expression of different cytokines. Relative expression of cytokines from one experiment is depicted in the figure. All the data is normalised with GAPDH expression. The significance was calculated by one-way ANOVA with Tukey’s multiple comparison. *p < 0.05, **p < 0.01, ***p < 0.001