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Fig. 6 | Cell & Bioscience

Fig. 6

From: Metabolomic profiling of Burkholderia pseudomallei using UHPLC-ESI-Q-TOF-MS reveals specific biomarkers including 4-methyl-5-thiazoleethanol and unique thiamine degradation pathway

Fig. 6

PCR for hydroxyethyl thiazole kinase genes and RT-PCR for mRNA detection in B. pseudomallei and B. thailandensis. a PCR of thiM from genomic DNA: thiM is present in the genomes of the three B. thailandensis strains, Bt1, Bt6 and Bt7, and 12 of 15 B. pseudomallei strains, but absent in the genomes of three B. pseudomallei strains, B24, B27 and VG550A (D10) with the highest 4-methyl-5-thiazoleethanol levels. b RT-PCR of thiM from mRNA of B. thailandensis: thiM is expressed in B. thailandensis strains Bt1, Bt6 and Bt7, but not expressed in the 12 B. pseudomallei strains that possessed thiM gene. Housekeeping gene apoB is used for normalization

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