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Fig. 5 | Cell & Bioscience

Fig. 5

From: Metabolomic profiling of Burkholderia pseudomallei using UHPLC-ESI-Q-TOF-MS reveals specific biomarkers including 4-methyl-5-thiazoleethanol and unique thiamine degradation pathway

Fig. 5

Function and phylogeny of thiaminase I and hydroxyethylthiazole kinase. a Thiaminase I (EC 2.5.1.2) catalyzes the degradation of thiamin by replacing the thiazole moiety with a variety of nucleophiles. Hydroxyethylthiazole kinase (EC 2.7.1.50) catalyzes the degradation of 4-methyl-5-thiazoleethanol by transferring the phosphorus-containing groups with an alcohol group as acceptor with ATP. b Phylogenetic tree showing the relationship of Thiaminase I in B. pseudomallei and B. thailandensis to homologues in other bacteria constructed by maximum-likelihood method. A total 341 amino acid positions were included in the analysis. Bootstrap values were calculated as percentages from 1000 replicates and only values ≥70 % were shown. The scale bars indicate the estimated number of substitutions per 5 amino acids. Names and accession numbers are given as cited in GenBank database. c Phylogenetic tree showing the relationship of hydroxyethylthiazole kinase in B. pseudomallei and B. thailandensis to homologues in other bacteria constructed by maximum-likelihood method. A total 261 amino acid positions were included in the analysis. Bootstrap values were calculated as percentages from 1000 replicates and only values ≥70 % were shown. The scale bars indicate the estimated number of substitutions per 5 amino acids. Names and accession numbers are given as cited in GenBank database

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