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Fig. 6 | Cell & Bioscience

Fig. 6

From: KLF13 promotes porcine adipocyte differentiation through PPARγ activation

Fig. 6

Transcriptional regulation of mouse key adipogenic factors by KLF13. a Effect of knockdown KLF13 on the expression of adipogenic factor genes during adipogenic differentiation of 3 T3-L1 cells. After 1 days transfection of KLF13 siRNA, adipose 3 T3-L1 were stimulated in adipogenic induction medium for 2 days. Real-time PCR was used to determine the mRNA expression of KLF13, KLF4, KLF5, Ebf1, KLF15, PPARγ2 and C/EBPα. Values are represented as mean ± SD. (n = 3). b The KLF13 mRNA level in overexpression of KLF13 during adipogenic differentiation of 3 T3-L1 cells. After 1 days transfection of pcDNA3.1-KLF13, 3 T3-L1 cells were stimulated in adipogenic induction medium for 2 days. Real-time PCR was used to determine the mRNA expression of KLF13. Values are represented as mean ± SD. (n = 3). c Effect of overexpression of KLF13 on the expression of adipogenic factor genes during adipogenic differentiation of 3 T3-L1 cells. After 1 days transfection of pcDNA3.1-KLF13, 3 T3-L1 cells were stimulated in adipogenic induction medium for 2 days. Real-time PCR was used to determine the mRNA expression of KLF4, KLF5, Ebf1, KLF15, PPARγ2 and C/EBPα. Values are represented as mean ± SD. (n = 3). d Effect of overexpression KLF13 on porcine PPARγ and mouse PPARγ2 promoters activity. Porcine PPARγ and mouse PPARγ2 promoter reporter were respectively co-transfected with pcDNA3.1-KLF13 into 3 T3-L1 cells. The dual-luciferase activity was measured 36 h after transfection. Values are represented as mean ± SD. (n = 3) *P < 0.05, **P < 0.01

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