Fig. 5

Expression and function of KLF13 in mouse 3 T3-L1 preadipocytes. a The mRNA expression of KLF13 in mouse 3 T3-L1 preadipocytes during adipocyte differentiation. The mRNA level was determined by real-time PCR and normalized to β-actin mRNA. The numbers indicate the time points of differentiation induction. Results are expressed as means ± SD. (n = 3). b The mRNA expression of PPARγ2 in mouse 3 T3-L1 preadipocytes during adipocyte differentiation. The mRNA level was determined by real-time PCR and normalized to β-actin mRNA. The numbers indicate the time points of differentiation induction. Results are expressed as means ± SD. (n = 3). c The KLF13 mRNA level in ASVC with the treatment of siRNA. siRNA was the siRNA sequences against mouse KLF13 (see Materials and Methods for details). Ctrol indicates control siRNA sequence. Endogenous KLF13 mRNA was determined by real-time PCR in pre-induction. Results are expressed as means ± SD. (n = 3). d Effect of knockdown KLF13 on adipogenic differentiation of 3 T3-L1 cells. 3 T3-L1 preadipocytes were treated with KLF13 siRNA at about 70 % confluence. After 24 h, the cells were induced to adipogenic differentiation. On day 8, the cell monolayer was stained with Oil-red O. e The mRNA expression of PPARγ, aP2 and Adiponectin in KLF13-knockdown 3 T3-L1 cells were detected by real-time PCR on day 8 after adipogenic induction. Results are expressed as means ± SD. (n = 3) *P < 0.05, **P < 0.01