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Fig. 3 | Cell & Bioscience

Fig. 3

From: KLF13 promotes porcine adipocyte differentiation through PPARγ activation

Fig. 3

Effect of KLF13 on the expression of PPARγ during adipogenic differentiation of porcine ASVC. a Analysis of transcription factor site within the PPARγ promoter. Genomatix MatInspector was used to identify transcription factor site within the promoter of PPARγ from 2000 bp upstream of the translation initiation site. b Effect of KLF13 knockdown on the expression of PPARγ during adipogenic differentiation of porcine ASVC. After 1 days transfection of KLF13 siRNA, SV cells were stimulated in adipogenic induction medium for 3 days. Western-blot was used to determine the protein expression of PPARγ. c Effect of KLF13 overexpression on the expression of PPARγ during adipogenic differentiation of porcine ASVC. After 1 days transfection of pcDNA3.1-KLF13, SV cells were stimulated in adipogenic induction medium for 3 days. Western-blot was used to determine the protein expression of PPARγ. Values are represented as mean ± SD. (n = 3) *P < 0.05

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