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Fig. 2 | Cell & Bioscience

Fig. 2

From: KLF13 promotes porcine adipocyte differentiation through PPARγ activation

Fig. 2

Effect of knockdown or overexpression of KLF13 on the expression of adipogenic factors. a After 2 days transfection of KLF13 siRNA, adipose SV cells were harvested. Real-time PCR was used to determine the mRNA expression of adipogenic factor genes Ebf1, KLF4, C/EBPβ, KLF15, PPARγ and C/EBPα. The protein level was determined by western blotting. Values are represented as mean ± SD. (n = 3). b After 2 days transfection of pcDNA3.1-KLF13, adipose SV cells were harvested. Real-time PCR was used to determine the mRNA expression of adipocyte differentiation-related genes Ebf1, KLF4, C/EBPβ, KLF15, PPARγ and C/EBPα. The protein level was determined by western blotting. Values are represented as mean ± SD. (n = 3). c After 1 days transfection of KLF13 siRNA, adipose SV cells were stimulated in adipogenic induction medium for 2 days. Real-time PCR was used to determine the mRNA expression of adipocyte differentiation-related genes Ebf1, KLF4, C/EBPβ, KLF9, KLF15, PPARγ and C/EBPα. The protein level was determined by western blotting. Values are represented as mean ± SD. (n = 3). d After 1 days transfection of pcDNA3.1-KLF13, adipose SV cells were stimulated in adipogenic induction medium for 2 days. Real-time PCR was used to determine the mRNA expression of KLF13. The protein level was determined by western blotting. Values are represented as mean ± SD. (n = 3). e After 1 days transfection of pcDNA3.1-KLF13, adipose SV cells were stimulated in adipogenic induction medium for 2 days. Real-time PCR was used to determine the mRNA expression of adipocyte differentiation-related genes Ebf1, KLF4, C/EBPβ, KLF15, PPARγ and C/EBPα. Values are represented as mean ± SD. (n = 3) *P < 0.05, **P < 0.01

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