Fig. 1

Expression and function of KLF13 in porcine ASVC. a The mRNA expression of KLF13 in porcine ASVC during adipocyte differentiation. The mRNA level was determined by real-time PCR and normalized to β-actin mRNA. The numbers indicate the time points of differentiation induction. Results are expressed as means ± SD. (n = 3) b The mRNA and protein expression of KLF13 in porcine ASVC during the early stages of adipocyte differentiation. The mRNA level was determined by real-time PCR and normalized to β-actin mRNA. The protein level was determined by western blotting. The numbers indicate the time points of differentiation induction. Results are expressed as means ± SD. (n = 3). c The mRNA and protein expression of KLF13 in ASVC with the treatment of siRNA. siRNA1, siRNA2, and siRNA3 were three siRNA sequences (see Materials and methods for details). Ctrol indicates control siRNA sequence. Endogenous KLF13 mRNA was determined by real-time PCR in pre-induction. The protein level was determined by western blotting. Results are expressed as means ± SD. (n = 3). d The KLF13 mRNA level in ASVC transiently transfected with pcDNA3.1-KLF13 or empty vector. Endogenous KLF13 mRNA was determined by real-time PCR in pre-induction. The protein level was determined by western blotting. Results are expressed as means ± SD. (n = 3). e Blocked ASVC adipocyte differentiation by KLF13 knockdown. ASVC were treated with KLF13 siRNA at about 70 % confluence. After 24 h, the cells were induced to adipogenic differentiation. On day 8, the cell monolayer was stained with Oil-red O. f The mRNA expression of PPARγ and aP2 in KLF13-knockdown ASVC were detected by real-time PCR on day 8 after adipogenic induction. Results are expressed as means ± SD. (n = 3). g Overexpression of KLF13 resulted in enhanced differentiation. ASVC were treated with KLF13 overexpression plasmid at about 70 % confluence. After 24 h, the cells were induced to adipogenic differentiation. On day 8, the cell monolayer was stained with Oil-red O. h The mRNA expression of PPARγ, aP2 and Adiponectin in KLF13-overexpression ASVC were detected by real-time PCR on day 8 after adipogenic induction. Results are expressed as means ± SD. (n = 3) *P < 0.05, **P < 0.01