Skip to main content

Advertisement

Table 2 Flow cytometry using annexin V-FITC demonstrating viable cells, cells in early apoptosis, cells in late apoptosis and necrotic cells

From: A 2-methoxyestradiol bis-sulphamoylated derivative induces apoptosis in breast cell lines

  Viable cells Cells occupying early apoptosis Cells occupying late apoptosis Necrotic cells
MCF-7 cells
Vehicle-treated cells 99.3% 0.3% 0.1% 0.3%
EMBS-treated cells 65.5%* 0.0% 22.21%* 12.3%
Actinomycin D-treated cells 49.3%* 0.0% 30.11%* 20.5%*
MDA-MB-231
Vehicle-treated cells 98.6% 0.0% 0.6% 0.9%
EMBS-treated cells 65.4%* 0.7% 21.6%* 12.3%
Actinomycin D-treated cells 48.4%* 9.3% 40.3%* 1.9%
MCF-12A cells
Vehicle-treated cells 98.6% 1.1% 0.0% 0.2%
EMBS-treated cells 66.13%* 17.4% 11.4% 5.1%
Actinomycin D-treated cells 46.57%* 32.71%* 17.6% 3.1%
  1. A * demonstrates a statistically significant P value of <0.05 when compared to vehicle-treated cells.