Table 2 Flow cytometry using annexin V-FITC demonstrating viable cells, cells in early apoptosis, cells in late apoptosis and necrotic cells
From: A 2-methoxyestradiol bis-sulphamoylated derivative induces apoptosis in breast cell lines
Viable cells | Cells occupying early apoptosis | Cells occupying late apoptosis | Necrotic cells | |
|---|---|---|---|---|
MCF-7 cells | ||||
Vehicle-treated cells | 99.3% | 0.3% | 0.1% | 0.3% |
EMBS-treated cells | 65.5%* | 0.0% | 22.21%* | 12.3% |
Actinomycin D-treated cells | 49.3%* | 0.0% | 30.11%* | 20.5%* |
MDA-MB-231 | ||||
Vehicle-treated cells | 98.6% | 0.0% | 0.6% | 0.9% |
EMBS-treated cells | 65.4%* | 0.7% | 21.6%* | 12.3% |
Actinomycin D-treated cells | 48.4%* | 9.3% | 40.3%* | 1.9% |
MCF-12A cells | ||||
Vehicle-treated cells | 98.6% | 1.1% | 0.0% | 0.2% |
EMBS-treated cells | 66.13%* | 17.4% | 11.4% | 5.1% |
Actinomycin D-treated cells | 46.57%* | 32.71%* | 17.6% | 3.1% |