Table 1 Flow cytometry employing propidium iodide and ethanol fixation demonstrating cell cycle progression
From: A 2-methoxyestradiol bis-sulphamoylated derivative induces apoptosis in breast cell lines
Sub G 1 | G 1 | S | G 2 M | |
|---|---|---|---|---|
MCF-7 cells | ||||
Vehicle-treated cells | 1.16% | 73.53% | 11.43% | 13.86% |
EMBS-treated cells | 36%* | 3.14% | 10.92% | 50%* |
Actinomycin D-treated cells | 62.7%* | 4.46% | 8.72% | 24.13% |
MDA-MB-231 | ||||
Vehicle-treated cells | 0.64% | 68.95% | 15.16% | 15.23% |
EMBS-treated cells | 27.9%* | 2.66% | 6.45% | 63%* |
Actinomycin D-treated cells | 71%* | 4.97% | 5.38% | 18.69% |
MCF-12A cells | ||||
Vehicle-treated cells | 0.78% | 68.33% | 12.42% | 18.45% |
EMBS-treated cells | 32.5%* | 4.14% | 11.69% | 51.7%* |
Actinomycin D-treated cells | 45.4%* | 4.06% | 9.85% | 40.7%* |