Figure 2
From: A 2-methoxyestradiol bis-sulphamoylated derivative induces apoptosis in breast cell lines
Effects of EMBS on cell cycle progression. Flow cytometrical investigation using propidium iodide and ethanol fixation demonstrated cell cycle progression. Cell cycle progression of vehicle-treated MCF-7 (A), MDA-MB-231 (B) and MCF-12A cells (C) revealed the majority of cells occupied the G1 phase and S phase, less than 2% of cells in the sub-G1 fraction, and 13-19% of cells occupying the G2M phase. Treated cells were exposed to 0.4 μM for 24 h. EMBS-treated MCF-7 (D), MDA-MB-231 (E) and MCF-12A cells (F) revealed a statistically significant increased sub-G1 peak and a statistically significant increased number of cells occupied the G2M phase. Statistical significance was indicated on 3 independent experiments each performed in triplicate with a paired student’s t-test. MCF-7 (G), MDA-MB-231 (H) and MCF-12A cells (I) exposed to 0.1 μg/ml actinomycin D used as a positive control for apoptosis induction resulted in apoptosis induction and an increase of cells occupying the G2M phase.